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Structural basis for coupling protein transport and N-glycosylation at the mammalian endoplasmic reticulum

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标题: Structural basis for coupling protein transport and N-glycosylation at the mammalian endoplasmic reticulum
资源摘要:

Protein synthesis, transport and N-glycosylation are coupled at the mammalian endoplasmic reticulum (ER) by complex formation between the ribosome, the Sec61 protein-conducting channel and the oligosaccharyltransferase (OST). Here, we used different cryo-electron microscopy approaches to determine structures of native and solubilized ribosome-Sec61-OST complexes. A molecular model for the catalytic OST subunit revealed how STT3A is integrated into the OST and how STT3 paralog specificity for translocon-associated OST is achieved. The OST subunit DC2 was placed at the interface between Sec61 and STT3A, where it acts as a versatile module for recruitment of STT3A-containing OST to the ribosome-Sec61 complex. This detailed structural view on the molecular architecture of the co-translational machinery for N-glycosylation provides the basis for a mechanistic understanding of glycoprotein biogenesis at the ER.

资源原始URL http://science.sciencemag.org/cgi/content/short/science.aar7899v1?rss=1
资源来源机构: Science
来源机构所属国家: 美国
来源机构性质: 科研

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